Nitric Oxide Synthase Detection Kit

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  • Published date: February 26, 2019
    • Shirley, New York

Diaminofluorescein -2 Diacetate (DAF-2DA) is a non-fluorescent cell permeable reagent that can measure free Nitric Oxide (NO) and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the diacetate groups on the DAF-2DA reagent are hydrolyze by cytosolic esterases thus releasing DAF-2 and sequestering the reagent inside the cell. Production of nitric oxide converts the nonfluorescent dye, DAF-2, to its fluorescent triaole derivative, DAF-2T. DAF-2T can be observed by: excitation 488nm and measuring emission at 515nm.

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Caroline Green
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