HTR5A U2OS β-Arrestin GPCR Assay Kit

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  • Published date: June 6, 2017
  • Modified date: September 20, 2018
    • United States

β-Arrestin GPCR cells are engineered to co-the ProLink (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
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