NOS Activity Assay Kit

  • Check with publisher
  • Published date: April 25, 2017
  • Modified date: August 27, 2018
    • United States

The NOS activity assay kit measures NOS activity by monitoring the conversion of radiolabeled arginine to citrulline. This assay is simple, sensitive, and specific for NOS activity and can be used with both crude and purified enzyme preparations. The kit includes sufficient materials and reagents for 50 total reactions. Radiolabeled arginine and NADPH are not included with the kit.For routine assays, radioactive arginine is added to protein extracts or purified NOS samples. After incubation, the reactions are stopped with a buffer containing ethylenediaminetetraacetic acid (EDTA), which chelates the calcium required by nNOS (neuronal NOS; NOS I) and eNOS (endothelial NOS; NOS III) and, consequently, inactivates the enzyme.
https://www.creativebiomart.net/nos-activity-assay-kit-463441.htm

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