MESG Phosphate Assay Kit

  • Check with publisher
  • Published date: May 30, 2019
    • Shirley, New York, United States

The MESG Phosphate Assay Kit has been developed for measuring the activity of any Pi-generating enzyme using MESG reagent. The measurement of Pi is based on absorbance change of MESG by phosphate. In the presence of inorganic phosphate, MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase with absorption wavelength shift to red. This feature has been used to develop our convenient MESG phosphate assay kit, an alternative to hazardous radioactive methods. The MESG substrate gives an absorbance increase at 360 nm on phosphorylysis at pH 6.5-8.5. The assay is shown to quantitate phosphate at the final concentration as low as 0.2 米M. The kit has been used for monitoring ATPase activities. It can also be used for monitoring phosphatase activities. Phosphate is involved in many biological processes. For example, phosphatases, ATPases and several other enzymes catalyze biochemical reactions in which inorganic phosphate (Pi) is released from a phosphoester substrate. The detection of many phosphoester每metabolizing enzymes is difficult because suitable substrates are not available. It is usually necessary to determine inorganic phosphate release using tedious colorimetric assays or radioisotope-based methods. http://www.creativebiomart.net/MESG-Phosphate-Assay-Kit-466765.htm

Caroline Green
0 votes

Useful information

  • Avoid scams by acting locally or paying with PayPal
  • Never pay with Western Union, Moneygram or other anonymous payment services
  • Don't buy or sell outside of your country. Don't accept cashier cheques from outside your country
  • This site is never involved in any transaction, and does not handle payments, shipping, guarantee transactions, provide escrow services, or offer "buyer protection" or "seller certification"

Related listings

  • NSD1 Chemiluminescent Assay Kit

    NSD1 Chemiluminescent Assay Kit

    Biological Sciences Shirley (New York) May 30, 2019 Check with publisher

    The NSD1 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD1 activity for screening and profiling applications. The key to the NSD1 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residu...

  • Recombinant HCV-2 Nonstructural Protein 5 (aa 2212-2313) [His]

    Recombinant HCV-2 Nonstructural Protein 5 (aa 2212-2313) [His]

    Biological Sciences Shirley (New York) May 30, 2019 Check with publisher

    Hepatitis C virus (HCV) is a member of the Flaviviridae family, genus hepacivirus. HCV is a small (55-65 nm in size), enveloped, positive-sense single-stranded RNA virus. E1 and E2 are two type 1 transmembrane glycoproteins that are essential compone...

  • Inactivated MuV Grade 3 Antigen

    Inactivated MuV Grade 3 Antigen

    Biological Sciences Shirley (New York) May 30, 2019 Check with publisher

    Mumps virus belongs to the genus Rubulavirus in the family Paramyxoviridae and produces roughly spherical, enveloped virions of about 200 nm in diameter. It is the causative agent of mumps, a well-known common childhood disease characterized by swell...