Recombinant Peptide Synthesis

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  • Published date: July 18, 2019
    • Shirley, New York, United States

For many years, chemical synthesis of large peptides and proteins has been a daunting task. The expansive progression in different ligation methods assists in the successful fulfillment of this large order. Since the building blocks can be obtained efficiently either by stepwise solid-phase synthetic or by recombinant methods, the limitation of size to produce synthetic or semisynthetic proteins is no longer a concern. Recombinant peptide is a manipulated form of peptide encoded by recombinant DNA, which has been cloned in a foreign expression system to supports the expression of the exogenous gene. This recombinant DNA construct can be used to manufacture large quantities of useful protein products. The recombinant DNA, usually the cDNA sequence of the target peptide, is designed to be under the control of a well-characterized promoter to express the target peptide within the chosen host cell to achieve high-level protein expression.

Cathy Miller
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