Parallel Artificial Membrane Permeability Assay (PAMPA)

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  • Published date: October 30, 2019
    • Shirley, New York, United States

Passive diffusion is an important way for drug absorption. It influences drugs pass through the intestinal epithelium, the blood brain barrier or transport across cell membranes. The PAMPA model is an artificial in vitro model suitable for the passive diffusion determination (passive permeability) of a compound. In PAMPA model, a lipid-infused artificial membrane is generated and acts as a supplement for cell-based assays. Test compound is added to multi-well microtitre plate in the donor compartment and permeating an artificial hexadecane membrane to the acceptor compartment. The result is quantified by LC-MS/MS. Lucifer yellow is used to assess membrane integrity. .

Jerry Carter
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