Time-Kill Analysis for Fungi

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  • Published date: January 15, 2020
    • Shirley, New York, United States

Time-kill curve assay is a broth-based method, and the rate of killing a fixed inoculum fungus is determined by comparing control group (no drug) and experiment group (antimicrobial agent-containing). The kill curves are constructed by plotting the CFU per milliliter surviving at each time point in the presence and absence of the antimicrobial agent. Kill curve of a single antimicrobial agent is often assessed and constructed by testing several multiples of the MIC. If determining synergy or antagonism effects between two or more antifungal drugs, kill curves are constructed for a fixed concentration of each agent alone and in combination and are compared to that of the no-drug control at each time point. https://www.creative-biolabs.com/drug-discovery/therapeutics/time-kill-analysis-for-fungi.htm .

Jerry Carter
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