Protease Substrates Screening Service

  • Check with publisher
  • Published date: April 20, 2017
  • Modified date: July 13, 2017
    • United States

Phage display peptide libraries that display random 5-20 mer peptides have been widely used to determine protease substrate [sequence] specificity for a large number of enzymes and semi-purified cell extracts. This technique has been playing a critical role in designing inhibitors and activators of proteases.

In comparison with phage display peptide libraries used in other fields, phage display peptide libraries used to identify the preferred substrate sequences of a protease usually express a fusion tag at the N-terminus of the peptide sequences, while, like in regular phage display peptide libraries, the minor coat viral protein pIII is located at the C-terminus of the peptide sequences. The N-terminal fusion tag is intended to bind the phage particles to a solid support. Scientists from Creative Biolabs have designed and built up a unique tag, HiAffi-tag, which is derived from Protein G, thus has strong binding affinity to most IgG coated on a solid surface. In contrast to His Tag and Strepavidin Binding Motifs used in such libraries, HiAffi-tag enables the best capture of the phage particles to the solid support and the least steric hindrance for proteolysis of the substrate peptides. More information, please visit the website: http://www.creative-biolabs.com/Protease-Substrate-Screening.html

Result 0 votes
Bella Smith
0 votes

Useful information

  • Avoid scams by acting locally or paying with PayPal
  • Never pay with Western Union, Moneygram or other anonymous payment services
  • Don't buy or sell outside of your country. Don't accept cashier cheques from outside your country
  • This site is never involved in any transaction, and does not handle payments, shipping, guarantee transactions, provide escrow services, or offer "buyer protection" or "seller certification"

Related listings

  • Screening for scFv/Fab with Excellent Thermal Stability

    Screening for scFv/Fab with Excellent Thermal Stability

    Immunology (United States ) April 20, 2017 Check with publisher

    Creative Biolabs offers specialized thermal stable antibody fragment (scFv/Fab) screening and antibody stability improvement services. With 10 years of experiences in antibody engineering and therapeutic antibody development, scientists in Creative B...

  • Internalizing Antibody Screening Service

    Internalizing Antibody Screening Service

    Immunology (United States ) April 20, 2017 Check with publisher

    Creative BioLabs offers high-throughput phage display library screening services for selection of receptor-dependent antibodies and peptides that can be internalized into mammalian cells. Internalizing antibodies and peptides specific for cell surfac...

  • Phage Display Library Screening Service

    Phage Display Library Screening Service

    Immunology (United States ) April 20, 2017 Check with publisher

    Our staff scientists have extensive experience in screening of phage display peptide, cDNA and scFv/Fab libraries. In particular, by conducting library screening, panning, for 4 cycles, we normally get scFv/Fab antibodies of an affinity of 10-7 By co...