Screening of Immunized Rabbit Antibody Library

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  • Published date: April 20, 2017
  • Modified date: July 13, 2017
    • United States

Selection of high affinity binders is mainly based on the in-solution phage display antibody library screening strategies we employ. We have two strategies: selection based on the equilibrium constant (Kd) and selection based on binding kinetics. In the first approach, sub-library phage is incubated with biotinylated antigen at controlled concentrations and bound phages are captured by immobilized NeutrAvidin. The second, selection based on binding kinetics is also termed off-rate (Koff) selection, in which phage population is allowed to saturate the labeled antigen before a large molar excess of unlabeled antigen is added to the mix for controlled periods of time. This allows the selection of mutant antibodies that have slower off-rates. Since a reduction in Koff usually results in a higher affinity, this selection approach singles out antibody variants with improved Kd. These methods allow selection of antibodies that have high affinity. In the end, the selected antibody fragments can be expressed to test their binding specificity and affinity.More information, please visit the website:

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Bella Smith
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