pull-down protein LC-MS

  • Check with publisher
  • Published date: January 20, 2020
    • Boston, Massachusetts, United States

"In the life of an organism, the function of proteins is achieved by the interaction between proteins and proteins. Therefore, the study of new proteins can provide new directions for the study of the potential function of proteins. In many protein tests, pull-down assays are often used to test protein-protein-interaction. Pull-down's experiment uses highly purified and enriched bait protein to capture the targeted proteins that specifically interact with the bait protein in the cell, and greatly improve the efficiency of identification of new target protein. The specific steps of pull-down's experiment are to express the bait protein modified with poly-His, or Biotin tags. Using immobilized affinity ligand to link the bait protein to the solid-phase matrix, and then mix the bait protein with the protein sample. In this process, targeted protein specifically interacts with the bait protein, and thus, the targeted proteins are isolated from the protein sample mixture. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Identification of the target protein is then processed by the mass spectrometer. Using CST pull-down assay kit, combined with advanced UPLC-MS platform, MtoZ Biolabs provides quick and accurate pull-down protein analysis service.


• Experiment procedures
• Parameters of liquid chromatography and mass spectrometer
• MS raw data files
• Peptide identifications and intensity
• Protein identifications and intensity"


Result 0 votes
Alex Hunt
0 votes

Useful information

  • Avoid scams by acting locally or paying with PayPal
  • Never pay with Western Union, Moneygram or other anonymous payment services
  • Don't buy or sell outside of your country. Don't accept cashier cheques from outside your country
  • This site is never involved in any transaction, and does not handle payments, shipping, guarantee transactions, provide escrow services, or offer "buyer protection" or "seller certification"

Related listings

  • CFAV H9-84 antigen

    CFAV H9-84 antigen

    Molecular Biology Shirley (New York) February 6, 2020 Check with publisher

    Cell fusing agent virus (CFAV) is an RNA insect virus that was isolated from a line of Aedes aegypti mosquito cells and has been assigned to the family Flaviviridae, genus Flavivirus. https://www.creative-diagnostics.com/CFAV-H9-84-antigen-228090-433...

  • Fowlpox virus antigen

    Fowlpox virus antigen

    Molecular Biology Shirley (New York) February 6, 2020 Check with publisher

    Fowlpox is the worldwide disease of poultry caused by viruses of the family Poxviridae and the genus Avipoxvirus. The viruses causing fowlpox are distinct from one another but antigenically similar, possible hosts including chickens, turkeys, quail, ...

  • PIV Antigen

    PIV Antigen

    Molecular Biology Shirley (New York) February 6, 2020 Check with publisher

    Parainfluenza viruses belong to the genus Paramyxovirus of the family Paramyxoviridae. They are enveloped viruses with a single-strand RNA genome of negative polarity and range in diameter from 150 to 200 nm. Four types of parainfluenza viruses have ...