SPR Surface plasmon resonance

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  • Published date: February 6, 2016
  • Modified date: February 7, 2016
    • Ankara, Turkey

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The theory is based on a physical process of light-metal film interaction. In SPR device, a material that has a high refractive index (with respect to medium) is coated with thin layer gold. When this gold coated material is treated with monochromatic light by a certain angle, some of the energy of light is transferred to free electrons of the gold and surface plasmons are formed on the gold. This plasmon will end up with specific refractive index. This phenomenon has been used for any molecular detection.

The application of SPR: On thin film gold surface, the molecule of interest is immobilized. This can be antibody, aptamer, cell, enzyme or etc. When the target molecule is sent to the media on top of molecule of interest and when association or dissociation happens between the immobilized olecule and target analyte, the refractive index will be changed which we can measured with high precision in real time by embedded sensors.

SPR technique has many advantages and label free detection is the most important advantage of it. SPR can be used to analyze biological or chemical materials and their molecular interactions without labeling molecules.

Real-Time analysis can be achieved with SPR techniques. Avidity and affinity of the samples can be detected. SPR technique offers almost at same level sensivity with ELISA and sometimes better.

Quantitave Analysis is an important feature with SPR devices. Every biological and chemical interaction can be monitored in details.

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Serhat Sevli
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