Uncovering the mechanisms of cryoprotectant toxicity and improving cryopreservation protocols

  • Check with publisher
  • Published date: May 15, 2016
  • Modified date: August 29, 2016
    • Liverpool , United Kingdom

Cryopreservation is the process of placing cells, organs or even whole organisms at low temperatures so that they remain in a state of suspended animation and function is preserved. Cryobiology is a crucial area of research for modern biotechnology due to the importance of biobanking, from developing reliable stem cell storage systems, organ banking for transplants as well as storage for engineered tissues. At present, cryopreservation technology is only successful for cell lines and very small tissues. More research is required before whole organs can successfully be cryopreserved while retaining their biological integrity. Given the importance of organ transplants and the growing field of tissue engineering, perfecting cryopreservation methods would have a profound impact on medicine.

We are looking for an enthusiastic and ambitious student to study cryopreservation and cryoprotectant toxicity. The constraints placed by delicate biological structures set many challenges for the science of cryopreservation. The chemicals which block ice formation and remove intracellular water (called cryoprotectants) are also toxic, to varying degrees. The complexity of this toxicity effect has been the biggest barrier to clinical application of vitrification in human tissues and organs. To unravel this complex sequence of interactions, we are using many techniques combined with genomic methods to study the mechanisms of cryoprotectant toxicity in human cells. Our goal is to identify genes and other mechanisms at work in cryoprotectant toxicity; this will give us direct targets for drug discovery. The exact direction of this project, however, will be adapted to fit the research interests of the student. Ultimately we aim to improve cryopreservation protocols to make long-term storage of stem cells, engineered tissues and organs more efficient.

Further details about our work on cryopreservation are available at:
http://cryopreservation.org.uk/

More information about our lab is also available online:
http://pcwww.liv.ac.uk/~aging/

Potential applicants are encouraged to contact Dr de Magalhaes in the first instance for an informal discussion.

Training associated with this project:
This project will provide a rich and diverse training in contemporary cell and molecular biology techniques, biochemistry, toxicology and cryobiology. Specifically, the student will be trained in cell and molecular biology techniques such as mammalian cell culture, qRT-PCR and RNAi.

This project is open to applicants who are able to obtain their own funding.

A fees bursary may be available for suitably qualified applicants.

Joao Pedro de Magalhaes
2 votes

Useful information

  • Avoid scams by acting locally or paying with PayPal
  • Never pay with Western Union, Moneygram or other anonymous payment services
  • Don't buy or sell outside of your country. Don't accept cashier cheques from outside your country
  • This site is never involved in any transaction, and does not handle payments, shipping, guarantee transactions, provide escrow services, or offer "buyer protection" or "seller certification"

Related listings

  • Exciting PhD Student Positions at IMPRS for Molecular Life Sciences, Munich

    Exciting PhD Student Positions at IMPRS for Molecular Life Sciences, Munich

    PhD Studentships Munich (Bavaria) September 19, 2017 Check with publisher

    The International Max Planck Research School for Molecular Life Sciences: From Biological Structures to Neural Circuits (IMPRS-LS) currently has an open call for competitive PhD student positions. IMPRS-LS brings together two renowned Max Planck Inst...

  • Developing drugs for the treatment of neurodegenerative diseases

    Developing drugs for the treatment of neurodegenerative diseases

    PhD Studentships Liverpool (United Kingdom) December 3, 2016 Check with publisher

    Ageing is arguably the major biomedical challenge of the 21st century with the incidence of age-related diseases, and neurodegenerative diseases in particular, expected to increase dramatically in the coming decades. In model organisms, the process o...

  • Novel pro-longevity compounds for the treatment of neurodegenerative diseases

    Novel pro-longevity compounds for the treatment of neurodegenerative diseases

    PhD Studentships Liverpool (United Kingdom) May 15, 2016 Check with publisher

    Ageing is arguably the major biomedical challenge of the 21st century with the incidence of age-related diseases, and neurodegenerative diseases in particular, expected to increase dramatically in the coming decades. In model organisms, caloric restr...